1,4-dibutyl-N-(2,6-dimethylphenyl)piperazine-2-carboxamide,nitrous acid
Jolkinolide B
CAS: 37905-08-1
Molecular Formula: C20H26O4
1,4-dibutyl-N-(2,6-dimethylphenyl)piperazine-2-carboxamide,nitrous acid - Names and Identifiers
1,4-dibutyl-N-(2,6-dimethylphenyl)piperazine-2-carboxamide,nitrous acid - Physico-chemical Properties
| Molecular Formula | C20H26O4 |
| Molar Mass | 330.42 |
| Density | 1.28±0.1 g/cm3 (20 ºC 760 Torr) |
| Melting Point | 213-215℃ |
| Boling Point | 497.1°C at 760 mmHg |
| Specific Rotation(α) | +220 (c, 0.4 in CHCl3) |
| Solubility | Very slightly soluble (0.36 g/L) (25 °C) |
| Appearance | Colorless needle crystal |
| Storage Condition | 2-8℃ |
1,4-dibutyl-N-(2,6-dimethylphenyl)piperazine-2-carboxamide,nitrous acid - Reference
| Reference Show more | 1. Li xiaolaga, O Wuliji, Wang Xiulan, et al. Study on HPLC fingerprint of Mongolian drug Tower Rinu and its different processed products [J]. Journal of Pharmaceutical Analysis, 2018, v.38(05):176-182. |
1,4-dibutyl-N-(2,6-dimethylphenyl)piperazine-2-carboxamide,nitrous acid - Reference Information
| overview | rock Euphorbium B(Jolkinolide B,JB) is the main component of Euphorbia chamaeformis. It was first published in Shennong's Materia Medica. It is used as a medicine and is poisonous. It is used by people to treat skin, tuberculosis, tumor and other diseases. The diterpenoid compound 17-hydroxy rock Euphorbium B isolated from the root of Euphorbia chamaejasme significantly inhibited the growth of K562 cells and induced their apoptosis in vitro, and found that it has significant anti-inflammatory effects. In addition, studies have shown that it can induce apoptosis of breast cancer MDA-MB-231 cells by inhibiting PI3K /Akt signaling pathway. It can be used for the treatment of malignant tumors. picture: wolf venom flower |
| source | rock Euphorbium lactone B is widely found in the moon gland Euphorbia and Euphorbia chamaedii, and the content of in Euphorbia chamaedii is relatively high, reaching 0.092 ~ 0.204%. |
| method for determination of content | wolfstella herb was extracted by ethanol ultrasonic after crushing, and the content of eupholide B was determined by high performance liquid phase chromatography. the chromatographic conditions are as follows: C18 column (250mm × 4.6 mm,5 μm), mobile phase: acetonitrile-water (65: 35), flow rate: 1.0 m L/mi, detection wavelength 238 nm, injection volume 10 μL, quantitative by external standard method. Results The linear relationship was good in the range of 4.070~32.560 μg/m L, r = 0.999 9, the average recovery rate was 98.02%, RS was 1.02%. Conclusion The method is specific, rapid and simple, and can be used as a method for the determination of content of Euphorbidione B in Euphorbia chamaedii, which can provide relevant basis for the formulation of quality standard of Euphorbia chamaedii. |
| anti-breast cancer cell mechanism | with the increase of eupholide B concentration, the proliferation of MDA-MB-231 cells can be significantly inhibited, and G1 phase cells block. Compared with the blank group, 20-40 μmol-1 Euphorbidione B inhibited the mRNA expression of Cyclin D1 and e IF4E, and the mRN expression and protein level of Cyclin D1 in the transfected IF4E-siRNA group were also inhibited, while the cell cycle was blocked in the G1 phase (P <0 01,P <0.01). Therefore, rock eupholide B can inhibit the proliferation of human breast cancer cells and arrest the cell cycle in G1 phase, which may be caused by inhibiting the expression of e IF4E and thus downregulating the expression of Cyclin D1. |
| anti-tumor mechanism | JB can inhibit the proliferation of human ovarian cancer Skov3 cells and promote cell apoptosis, which may play an anti-tumor effect by inhibiting PI3K/Akt signaling pathway and inducing death receptor expression. |
| references | [1] Lin Yu, Yue Liling, Jiang Liyan. Effect of Euphorbidide B on Proliferation of Human Breast Cancer MDA-MB-231 Cells [J]. Chinese Journal of Experimental Presentation, 2016(3):132-136. [2] Zhou Shanshan, xu Zongbin, Lu Wei. Inhibitory Effect of Euphorbidione B on Proliferation of Human Ovarian Cancer Skov3 Cells [J]. Chinese Journal of Biochemical Drugs, 2012, 33(5):567-570. [3] Wang Canjian, Yan Xiaohong, Xie Yanhui, et al. Determination of Euphorbione B Content in Euphorbione Chinensis [J]. New Chinese Medicine and Clinical Pharmacology, 2010, 21(5):521-524. |
Last Update：2024-04-09 20:52:54
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Product Name: Jolkinolide B Visit Supplier Webpage Request for quotationCAS: 37905-08-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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